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| Registros recuperados : 36 | |
| Registros recuperados : 36 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
12/04/2018 |
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Data da última atualização: |
08/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MOREIRA, R. F.; MATOS, M. N. C.; ALVES FILHO, J. G.; VALLE, R. V. do; ELOY, A. M. X.; PINTO, T. M. F.; MACHADO, S. P.; COSTA, C. R. R.; LIMA FILHO, J. L. de; LIMA, J. P. M. S.; CUNHA, R. M. S. da. |
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Afiliação: |
Raulzito Fernandes Moreira, Pós-graduação - Universidade Federal do Ceará (UFC) - Sobral, CE, Brasil.; Maria Nágila Carneiro Matos; João Garcia Alves Filho; Roberta Vianna do Valle; ANGELA MARIA XAVIER ELOY, CNPC; Tatiana Maria Farias Pinto; Saris Pinto Machado Junior; Cíntia Renata Rocha Costa; José Luiz de Lima Filho; João Paulo Matos Santos Lima; Rodrigo Maranguape Silva da Cunha. |
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Título: |
Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Animal Reproduction, Belo Horizonte, v. 15, n. 1, p. 84-92, jan./mar. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations. |
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Palavras-Chave: |
Análise proteômica; Isolation methods; Proteomic analysis; Proteomic profiles; Raça Moxotó. |
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Thesagro: |
Caprino; Detergente. |
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Thesaurus NAL: |
detergents; Goats; proteomics; Spermatozoa; Surfactants. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/175677/1/CNPC-Diversity-2018-.pdf
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Marc: |
LEADER 02033naa a2200385 a 4500
001 2090515
005 2019-04-08
008 2018 bl uuuu u00u1 u #d
100 1 $aMOREIRA, R. F.
245 $aDiversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods.$h[electronic resource]
260 $c2018
520 $aAbstract: This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations.
650 $adetergents
650 $aGoats
650 $aproteomics
650 $aSpermatozoa
650 $aSurfactants
650 $aCaprino
650 $aDetergente
653 $aAnálise proteômica
653 $aIsolation methods
653 $aProteomic analysis
653 $aProteomic profiles
653 $aRaça Moxotó
700 1 $aMATOS, M. N. C.
700 1 $aALVES FILHO, J. G.
700 1 $aVALLE, R. V. do
700 1 $aELOY, A. M. X.
700 1 $aPINTO, T. M. F.
700 1 $aMACHADO, S. P.
700 1 $aCOSTA, C. R. R.
700 1 $aLIMA FILHO, J. L. de
700 1 $aLIMA, J. P. M. S.
700 1 $aCUNHA, R. M. S. da
773 $tAnimal Reproduction, Belo Horizonte$gv. 15, n. 1, p. 84-92, jan./mar. 2018.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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